The Resource Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos, by Bethany Kay Bauer, (electronic resource)

Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos, by Bethany Kay Bauer, (electronic resource)

Label
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
Title
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
Statement of responsibility
by Bethany Kay Bauer
Title variation
Transcriptional profiling by deep sequencing identifies differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos cultured in vitro. Gilts were artificially inseminated and on Day 2 one oviduct and the tip of a uterine horn were flushed and the recovered embryos were cultured in PZM3 for four days. On Day 6 the gilts were euthanized and the contra-lateral horn was flushed to obtain in vivo derived embryos. Total RNA was extracted from 3 pools of 10 blastocysts from each treatment. First and second strand cDNA was synthesized and then sequenced using Illumina sequencing. The reads were aligned to a custom-built database designed to represent the known porcine "transcriptome". A total of 1,170 database members were different between the two groups (P[less than]0.05), and 588 of those were at least 2-fold different. Eleven transcripts were subjected to real-time PCR and each validated the sequencing. There was an overall decrease in both inner cell mass and trophectodermal cell numbers in embryos cultured in vitro, however, no difference in ICM:TE ratio was found. Interestingly, the transcript (SLC7A1) was higher in the in vitro cultured group. This difference disappeared after addition of arginine (0.36 mM) to the 4 day culture. In conclusion, Illumina sequencing and alignment to a custom "transcriptome" successfully identified a large number of genes that yield clues to the derivation of culture media
Cataloging source
MUU
http://library.link/vocab/creatorName
Bauer, Bethany Kay
Degree
M.S.
Dissertation year
2010.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Prather, Randall S.
http://library.link/vocab/subjectName
  • Swine
  • Swine
  • Fertilization in vitro
  • Ovum implantation
  • Genetic transcription
  • Nucleotide sequence
Target audience
specialized
Label
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos, by Bethany Kay Bauer, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on June 3, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. Randall Prather
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
646494863
Extent
1 online resource (xi, 88 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)646494863
Label
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos, by Bethany Kay Bauer, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on June 3, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. Randall Prather
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
646494863
Extent
1 online resource (xi, 88 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)646494863

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