The Resource Spermatogenesis : laser microdissection optimization, by Deborah A. Hansen

Spermatogenesis : laser microdissection optimization, by Deborah A. Hansen

Label
Spermatogenesis : laser microdissection optimization
Title
Spermatogenesis
Title remainder
laser microdissection optimization
Statement of responsibility
by Deborah A. Hansen
Title variation
Laser microdissection optimization
Creator
Subject
Genre
Language
eng
Summary
Studies link paternal environmental exposures to pesticides, herbicides, and radiation to adverse reproductive outcomes such as developmental delays, malignancies, and structural birth defects. To date, 900 children of Vietnam veterans with presumed Agent Orange exposure receive VA presumptive service connected benefits because these children have Spina Bifida. To date, there are limited data to explain the underlying causal mechanisms whereby paternal environmental exposures result in adverse reproductive events. Laser microdissection (LMD) is the only technology that allows the isolation of cell subpopulations from complex tissues such as the testes without disturbing the cell's bimolecular signature. LMD is essential in the comparison of the molecular signatures of diseased and non diseased cells. As a result, LMD will translate to toxicology protocols that seek to elucidate gene expression data from exposed and non exposed populations. The purpose of this study was to a) demonstrate the reliability of the sequential methods of LMD and the selected downstream applications through the establishment of qualitative and quantitative checkpoints upon which each subsequent step was based, b) ensure that the expected differences in gene expression during spermatogenesis can be characterized with LMD, and c) validate the LMD methods prior to proceeding to animal exposures. This study used the Leica LMD 6000 combined with qRT-PCR to generate stage specific gene expression data during spermatogenesis. Cluster LMD was used to maximize RNA yield and obtain pure stage specific cell populations. qRT-PCR was used to validate stage specific LMD success. With the exception of non specific mMagea4 amplification across all cell stages, the 10 fold rise in HspA2 in spermatocytes, and the 12 fold increase in Tnp1 and the 10 fold increase in prm2 in elongated spermatids supported stage specific LMD success. Cyp1A1 was found in spermatogonia, but was absent in spermatocytes and spermatids. These expression patterns paralleled the published data and coincided with the physiologic functions of these genes and the blood testes barrier
Member of
Cataloging source
UMS
http://library.link/vocab/creatorName
Hansen, Deborah A.,
Degree
Ph.D.
Dissertation year
2010.
Granting institution
University of Missouri--St. Louis
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Spermatogenesis
  • Spermatogenesis
  • Abnormalities, Human
  • Microdissection
  • Lasers
Label
Spermatogenesis : laser microdissection optimization, by Deborah A. Hansen
Instantiates
Publication
Note
Title from title page of PDF (University of Missouri--St. Louis, viewed March 17, 2011)
Bibliography note
Includes bibliographical references (pages 114-132)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
707634588
Extent
1 online resource (xii, 140 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)707634588
Label
Spermatogenesis : laser microdissection optimization, by Deborah A. Hansen
Publication
Note
Title from title page of PDF (University of Missouri--St. Louis, viewed March 17, 2011)
Bibliography note
Includes bibliographical references (pages 114-132)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
707634588
Extent
1 online resource (xii, 140 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)707634588

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