The Resource Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation, by Luxin Wang, (electronic resource)

Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation, by Luxin Wang, (electronic resource)

Label
Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation
Title
Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation
Statement of responsibility
by Luxin Wang
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
The objectives of this study were to establish a real-time multiplex polymerase chain reaction (PCR) for simultaneous quantitation of Escherichia coli O157:H7, Salmonella and Shigella that have been implicated in a number of foodborne disease outbreaks. Genomic DNA for the real-time PCR was extracted by the boiling method. Three sets of primers and corresponding TaqMan® probes were designed to target these three pathogens. Multiplex real-time PCR was carried out with TaqMan® Universal PCR Master Mix in an ABI Prism 7700 Sequence Detection System. Final standard curves were calculated by plotting the threshold cycle (Ct) value against log10 CFU/ml by linear regression to analyze the results for each pathogen. With optimized conditions, the quantitative detection ranges of the real-time multiplex PCR for pure cultures were 102 to 109 CFU/ml for E. coli O157:H7, 103 to 109 CFU/ml for Salmonella and 101 to 108 CFU/ml for Shigella. When this established multiplex real-time PCR system was applied to ground beef samples, the lowest detection concentration of three pathogens were increased to 105 CFU/g for E. coli O157:H7, 103 CFU/g for Salmonella and 104 CFU/g for Shigella. Immunomagnetic separation was then used to isolate E. coli O157:H7 and Salmonella from the beef samples. The lowest detection concentrations of three pathogens were reduced to 103 CFU/g. TaqMan® real-time PCR, combined with IMS has the potential to be a faster and more reliable method for rapid quantitation of E. coli O157:H7, Salmonella and Shigella in food, which will take 3 h for the whole process
Cataloging source
MUU
http://library.link/vocab/creatorDate
1983-
http://library.link/vocab/creatorName
Wang, Luxin
Degree
M.S.
Dissertation year
2006.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Mustapha, Azlin
http://library.link/vocab/subjectName
  • Polymerase chain reaction
  • Food contamination
  • Escherichia coli O157:H7
  • Salmonella
  • Shigella
Target audience
specialized
Label
Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation, by Luxin Wang, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (February 23, 2007)
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
84914118
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)84914118
System details
Mode of access: World Wide Web
Label
Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation, by Luxin Wang, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (February 23, 2007)
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
84914118
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)84914118
System details
Mode of access: World Wide Web

Library Locations

  • Health Sciences LibraryBorrow it
    2411 Holmes St, Kansas City, Kansas City, MO, 64108, US
    39.083418 -94.575323
  • LaBudde Special CollectionsBorrow it
    800 E 51st St, Kansas City, MO, 64110, US
    39.034642 -94.576835
  • Leon E. Bloch Law LibraryBorrow it
    500 E. 52nd Street, Kansas City, MO, 64110, US
    39.032488 -94.581967
  • Marr Sound ArchivesBorrow it
    800 E 51st St, Kansas City, MO, 64110, US
    39.034642 -94.576835
  • Miller Nichols LibraryBorrow it
    800 E 51st St, Kansas City, MO, 64110, US
    39.035061 -94.576518
  • UMKCBorrow it
    800 E 51st St, Kansas City, MO, 64110, US
    39.035061 -94.576518
  • UMKCBorrow it
    800 E 51st St, Kansas City, MO, 64110, US
Processing Feedback ...