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The Resource PCR sequencing protocols, edited by Ralph Rapley

PCR sequencing protocols, edited by Ralph Rapley

Label
PCR sequencing protocols
Title
PCR sequencing protocols
Statement of responsibility
edited by Ralph Rapley
Contributor
Subject
Genre
Language
eng
Member of
Cataloging source
DLC
Dewey number
574.87/3283
Illustrations
illustrations
Index
index present
LC call number
QP606.D46
LC item number
P365 1996
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
v.65
Nature of contents
bibliography
http://library.link/vocab/relatedWorkOrContributorName
Rapley, Ralph
Series statement
Methods in molecular biology
Series volume
v. 65
http://library.link/vocab/subjectName
  • Polymerase chain reaction
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Polymerase Chain Reaction
Label
PCR sequencing protocols, edited by Ralph Rapley
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preparation and analysis of DNA sequencing gels -- Purification of PCR products from agarose gels for direct sequencing -- Enzymatic fluorescence and biotin labeling of primers for PCR sequencing -- Direct sequencing of double-stranded PCR products with the sequenase kit and [alpha-35S] dATP -- Direct sequencing by thermal asymmetric PCR -- Rapid sequencing of cDNA clones : direct sequencing using sequential linear/asymmetric PCR -- Direct sequencing of PCR products using chemiluninescent detection -- Direct DNA sequencing of PCR products using magnetic beads -- Affinity capture and solid-phase sequencing of biotinylated PCR products -- Analysis of nucleotide sequence variations by solid-phase minisequencing -- Nonradioactive PCR sequencing using digoxigenin -- Silver Sequencing nonradioactive cycle sequencing of dsDNA -- Direct sequencing of PCR products with DNA-binding proteins -- PCR sequencing with the aid of detergents -- Direct sequencing with highly degenerate and inosine-containing primers -- Determination of unknown genomic sequences without cloning -- DNA sequencing by the chemical method -- Direct PCR sequencing with denaturants (formamide) -- Efficient PCR production of single-stranded DNA sequencing templates -- Preparation and direct automated cycle sequencing of PCR products -- Solid-phase automated sequencing of PCR-ampilified genomic DNA -- Cloning PCR products for sequencing in M13 vectors -- Sequencing PCR products cloned into M13 vectors -- Genomic ampilificatin with transcript sequencing (GAWTS) -- DNA rescue by the vectorette method -- Sequencing of (dA:dT) cloned mixed PCR products from microbial populations
Control code
35262236
Dimensions
23 cm
Extent
xi, 221 pages
Isbn
9780896033443
Isbn Type
(acid-free paper)
Lccn
96027724
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
Label
PCR sequencing protocols, edited by Ralph Rapley
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preparation and analysis of DNA sequencing gels -- Purification of PCR products from agarose gels for direct sequencing -- Enzymatic fluorescence and biotin labeling of primers for PCR sequencing -- Direct sequencing of double-stranded PCR products with the sequenase kit and [alpha-35S] dATP -- Direct sequencing by thermal asymmetric PCR -- Rapid sequencing of cDNA clones : direct sequencing using sequential linear/asymmetric PCR -- Direct sequencing of PCR products using chemiluninescent detection -- Direct DNA sequencing of PCR products using magnetic beads -- Affinity capture and solid-phase sequencing of biotinylated PCR products -- Analysis of nucleotide sequence variations by solid-phase minisequencing -- Nonradioactive PCR sequencing using digoxigenin -- Silver Sequencing nonradioactive cycle sequencing of dsDNA -- Direct sequencing of PCR products with DNA-binding proteins -- PCR sequencing with the aid of detergents -- Direct sequencing with highly degenerate and inosine-containing primers -- Determination of unknown genomic sequences without cloning -- DNA sequencing by the chemical method -- Direct PCR sequencing with denaturants (formamide) -- Efficient PCR production of single-stranded DNA sequencing templates -- Preparation and direct automated cycle sequencing of PCR products -- Solid-phase automated sequencing of PCR-ampilified genomic DNA -- Cloning PCR products for sequencing in M13 vectors -- Sequencing PCR products cloned into M13 vectors -- Genomic ampilificatin with transcript sequencing (GAWTS) -- DNA rescue by the vectorette method -- Sequencing of (dA:dT) cloned mixed PCR products from microbial populations
Control code
35262236
Dimensions
23 cm
Extent
xi, 221 pages
Isbn
9780896033443
Isbn Type
(acid-free paper)
Lccn
96027724
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations

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