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The Resource PCR cloning protocols : from molecular cloning to genetic engineering, edited by Bruce A. White

PCR cloning protocols : from molecular cloning to genetic engineering, edited by Bruce A. White

Contributor
Summary
This book provides extensive protocols on the use of PCR for molecular cloning, including the cloning of known sequences, cloning of unknown sequences and cloning of gene family members
Language
eng
Work
Publication
Extent
xiv, 490 pages
Contents
  • PCR : basic principles and routine practice / Lori A. Kolmodin, J. Fenton Williams
  • XL PCR amplification of long targets from genomic DNA / Suzanne Cheng, Lori A. Kolmodin
  • Amplification of DNA sequences up to 5 kb from small amounts of genomic DNA using tub DNA polymerase / Helen B. Forrester, Ian R. Radford
  • One-step optimization using touchdown and stepdown PCR / Kenneth H. Roux, Karl H. Hecker
  • GC-rich template amplification by inverse PCR : DNA polymerase and solvent effects / Alain Moreau, Colette Duez, Jean Dusart
  • Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / Jyrki T. Aatsinki
  • Using T4 DNA polymerase to generate clonable PCR products / Kai Wang
  • Rapid (ligase-free) subcloning of PCR products / Alan R. Shuldiner, Keith Tanner
  • Cloning PCR products utilizing the T/A overhang and a kit / Melissa Lail-Trecker
  • Cloning unmodified PCR products using engineered Xcml restriction sites in a portable cassette / Alessandro Testori, Paul Sollitti
  • A T-linker strategy for modification and directional cloning of PCR products / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine
  • Recovery of DNA amplification products from silver-stained polyacrylamide gels : applications in nucleic acid fingerprinting and genetic mapping / Gustavo Caetano-Anollés, Robert N. Trigiano
  • Recombination and site-directed mutagenesis using recombination PCR / Douglas H. Jones, Stanley C. Winistorfer
  • In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton
  • In-frame cloning of synthetic genes using PCR inserts / James C. Pierce
  • Creation of chimeric junctions, deletions, and insertions by PCR / Geneviève Pont-Kingdon
  • Mutagenesis and gene fusion by megaprimer PCR / Sailen Barik
  • Rapid and efficient one-tube PCR-based mutagenesis method / Veronique Picard, Susan Clark Bock
  • Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification / Scott F. Michael
  • Linker scanning mutagenesis by three-step PCR / Judith T. Schanke
  • Sequence inversion by flip-PCR / Judith T. Schanke
  • PCR site-directed mutagenesis using pyrococcus sp GB-D polymerase coupled to a rapid screening procedure : application to a [beta]-glucanase gene / Jaume Pons, Antoni Planas, Miquel Juncosa, Enrique Querol
  • Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules / Craig Tuerk
  • Rapid amplification of cDNA ends / David Bertioli
  • Amplification of gene-regulating regions with single-sided specificity / Mei-Zhong Luo, Rino Cella
  • An end-trimming method and its application to amplify adjacent cDNA and genomic DNA fragments by PCR / Hiroyuki Iwahana, Mitsuo Itakura
  • Anchoring a defined sequence to the 5' ends of mRNAs : the bolt to clone rare full-length mRNAs / Jean Baptiste Edwards, Olivier Valdenaire, Jacques Mallet
  • Rapid directional walk within DNA clones by step-out PCR / Umadevi V. Wesley, Cedric S. Wesley
  • Inverse PCR : an efficient approach to cloning cDNA ends / Sheng-He Huang
  • Rapid amplification of gene ends (RAGE) from gene libraries by anchored PCR / Sheng-He Huang, Ambrose Y. Jong
  • Isolation of coding sequences from yeast artificial chromosome (Yac) : clones by exon amplification / Fernando Gibson, Steve D. Brown
  • cDNA libraries from a low amount of cells / Philippe Ravassard, Christine Icard-Liepkalns, Jacques Mallet, Jean Baptiste Edwards
  • Rapid and nonradioactive screening of recombinant libraries by PCR / Michael W. King
  • Use of PCR for cDNA library screening / Toru Takumi
  • Generation and PCR screening of bacteriophage [lambda] sublibraries enriched for rare clones (the "sublibrary method") / Michael Lardelli
  • Normalization of cDNA sequence representation by molecular selection / Thierry G. Coche
  • Subtractive cDNA cloning using magnetic beads and PCR / Thierry G. Coche
  • Generation of a PCR-renewable source of subtractive cDNA / W. Michael Kuehl, James Battey
  • The use of PCR for differential screening of cDNA libraries / Mark G. Thomas, Sarah A. Hesse, Yvonne J. Foss, Farzin Farzaneh
  • Identification and cloning of differentially expressed genes by DDRT-PCR / Mikkel Rohde, Rene Hummel, Niels Pallisgaard, Tino Podstufka, Heidi Riedel, Henrik Leffers, Michael Strauss
  • Cloning gene family members using PCR with degenerate oligonucleotide primers / Gregory M. Preston
  • Amplification using degenerate primers with multiple inosines to isolate genes with minimal sequence similarity / Simona Bartl
  • Designing PCR primers to amplify specific members or subgroups of multigene families / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine
  • Screening gene family-enriched cDNA sublibraries with an unamplified cDNA probe : focusing on moderately to abundantly expressed clones / Meimei Hu, Bruce A. White
Isbn
9780896034839
Instance
Label
PCR cloning protocols : from molecular cloning to genetic engineering
Title
PCR cloning protocols
Title remainder
from molecular cloning to genetic engineering
Statement of responsibility
edited by Bruce A. White
Contributor
Subject
Genre
Language
eng
Summary
This book provides extensive protocols on the use of PCR for molecular cloning, including the cloning of known sequences, cloning of unknown sequences and cloning of gene family members
Member of
Cataloging source
DLC
Dewey number
574.87/3282
Illustrations
illustrations
Index
index present
LC call number
QH442.2
LC item number
.P37 1997
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
v.67
Nature of contents
bibliography
NLM call number
  • W1
  • QH442.2 .P348 1997
NLM item number
ME9616J v.67 1997
http://library.link/vocab/relatedWorkOrContributorName
White, Bruce Alan
Series statement
Methods in molecular biology
Series volume
vol. 67
http://library.link/vocab/subjectName
  • Molecular cloning
  • Polymerase chain reaction
  • Molecular cloning
  • Cloning, Molecular
  • Polymerase Chain Reaction
Label
PCR cloning protocols : from molecular cloning to genetic engineering, edited by Bruce A. White
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
PCR : basic principles and routine practice / Lori A. Kolmodin, J. Fenton Williams -- XL PCR amplification of long targets from genomic DNA / Suzanne Cheng, Lori A. Kolmodin -- Amplification of DNA sequences up to 5 kb from small amounts of genomic DNA using tub DNA polymerase / Helen B. Forrester, Ian R. Radford -- One-step optimization using touchdown and stepdown PCR / Kenneth H. Roux, Karl H. Hecker -- GC-rich template amplification by inverse PCR : DNA polymerase and solvent effects / Alain Moreau, Colette Duez, Jean Dusart -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / Jyrki T. Aatsinki -- Using T4 DNA polymerase to generate clonable PCR products / Kai Wang -- Rapid (ligase-free) subcloning of PCR products / Alan R. Shuldiner, Keith Tanner -- Cloning PCR products utilizing the T/A overhang and a kit / Melissa Lail-Trecker -- Cloning unmodified PCR products using engineered Xcml restriction sites in a portable cassette / Alessandro Testori, Paul Sollitti -- A T-linker strategy for modification and directional cloning of PCR products / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Recovery of DNA amplification products from silver-stained polyacrylamide gels : applications in nucleic acid fingerprinting and genetic mapping / Gustavo Caetano-Anollés, Robert N. Trigiano -- Recombination and site-directed mutagenesis using recombination PCR / Douglas H. Jones, Stanley C. Winistorfer -- In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton -- In-frame cloning of synthetic genes using PCR inserts / James C. Pierce -- Creation of chimeric junctions, deletions, and insertions by PCR / Geneviève Pont-Kingdon -- Mutagenesis and gene fusion by megaprimer PCR / Sailen Barik -- Rapid and efficient one-tube PCR-based mutagenesis method / Veronique Picard, Susan Clark Bock -- Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification / Scott F. Michael -- Linker scanning mutagenesis by three-step PCR / Judith T. Schanke -- Sequence inversion by flip-PCR / Judith T. Schanke -- PCR site-directed mutagenesis using pyrococcus sp GB-D polymerase coupled to a rapid screening procedure : application to a [beta]-glucanase gene / Jaume Pons, Antoni Planas, Miquel Juncosa, Enrique Querol -- Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules / Craig Tuerk -- Rapid amplification of cDNA ends / David Bertioli -- Amplification of gene-regulating regions with single-sided specificity / Mei-Zhong Luo, Rino Cella -- An end-trimming method and its application to amplify adjacent cDNA and genomic DNA fragments by PCR / Hiroyuki Iwahana, Mitsuo Itakura -- Anchoring a defined sequence to the 5' ends of mRNAs : the bolt to clone rare full-length mRNAs / Jean Baptiste Edwards, Olivier Valdenaire, Jacques Mallet -- Rapid directional walk within DNA clones by step-out PCR / Umadevi V. Wesley, Cedric S. Wesley -- Inverse PCR : an efficient approach to cloning cDNA ends / Sheng-He Huang -- Rapid amplification of gene ends (RAGE) from gene libraries by anchored PCR / Sheng-He Huang, Ambrose Y. Jong -- Isolation of coding sequences from yeast artificial chromosome (Yac) : clones by exon amplification / Fernando Gibson, Steve D. Brown -- cDNA libraries from a low amount of cells / Philippe Ravassard, Christine Icard-Liepkalns, Jacques Mallet, Jean Baptiste Edwards -- Rapid and nonradioactive screening of recombinant libraries by PCR / Michael W. King -- Use of PCR for cDNA library screening / Toru Takumi -- Generation and PCR screening of bacteriophage [lambda] sublibraries enriched for rare clones (the "sublibrary method") / Michael Lardelli -- Normalization of cDNA sequence representation by molecular selection / Thierry G. Coche -- Subtractive cDNA cloning using magnetic beads and PCR / Thierry G. Coche -- Generation of a PCR-renewable source of subtractive cDNA / W. Michael Kuehl, James Battey -- The use of PCR for differential screening of cDNA libraries / Mark G. Thomas, Sarah A. Hesse, Yvonne J. Foss, Farzin Farzaneh -- Identification and cloning of differentially expressed genes by DDRT-PCR / Mikkel Rohde, Rene Hummel, Niels Pallisgaard, Tino Podstufka, Heidi Riedel, Henrik Leffers, Michael Strauss -- Cloning gene family members using PCR with degenerate oligonucleotide primers / Gregory M. Preston -- Amplification using degenerate primers with multiple inosines to isolate genes with minimal sequence similarity / Simona Bartl -- Designing PCR primers to amplify specific members or subgroups of multigene families / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Screening gene family-enriched cDNA sublibraries with an unamplified cDNA probe : focusing on moderately to abundantly expressed clones / Meimei Hu, Bruce A. White
Control code
35192222
Dimensions
24 cm
Extent
xiv, 490 pages
Isbn
9780896034839
Lccn
96030876
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
System control number
(OCoLC)35192222
Label
PCR cloning protocols : from molecular cloning to genetic engineering, edited by Bruce A. White
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
PCR : basic principles and routine practice / Lori A. Kolmodin, J. Fenton Williams -- XL PCR amplification of long targets from genomic DNA / Suzanne Cheng, Lori A. Kolmodin -- Amplification of DNA sequences up to 5 kb from small amounts of genomic DNA using tub DNA polymerase / Helen B. Forrester, Ian R. Radford -- One-step optimization using touchdown and stepdown PCR / Kenneth H. Roux, Karl H. Hecker -- GC-rich template amplification by inverse PCR : DNA polymerase and solvent effects / Alain Moreau, Colette Duez, Jean Dusart -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / Jyrki T. Aatsinki -- Using T4 DNA polymerase to generate clonable PCR products / Kai Wang -- Rapid (ligase-free) subcloning of PCR products / Alan R. Shuldiner, Keith Tanner -- Cloning PCR products utilizing the T/A overhang and a kit / Melissa Lail-Trecker -- Cloning unmodified PCR products using engineered Xcml restriction sites in a portable cassette / Alessandro Testori, Paul Sollitti -- A T-linker strategy for modification and directional cloning of PCR products / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Recovery of DNA amplification products from silver-stained polyacrylamide gels : applications in nucleic acid fingerprinting and genetic mapping / Gustavo Caetano-Anollés, Robert N. Trigiano -- Recombination and site-directed mutagenesis using recombination PCR / Douglas H. Jones, Stanley C. Winistorfer -- In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton -- In-frame cloning of synthetic genes using PCR inserts / James C. Pierce -- Creation of chimeric junctions, deletions, and insertions by PCR / Geneviève Pont-Kingdon -- Mutagenesis and gene fusion by megaprimer PCR / Sailen Barik -- Rapid and efficient one-tube PCR-based mutagenesis method / Veronique Picard, Susan Clark Bock -- Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification / Scott F. Michael -- Linker scanning mutagenesis by three-step PCR / Judith T. Schanke -- Sequence inversion by flip-PCR / Judith T. Schanke -- PCR site-directed mutagenesis using pyrococcus sp GB-D polymerase coupled to a rapid screening procedure : application to a [beta]-glucanase gene / Jaume Pons, Antoni Planas, Miquel Juncosa, Enrique Querol -- Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules / Craig Tuerk -- Rapid amplification of cDNA ends / David Bertioli -- Amplification of gene-regulating regions with single-sided specificity / Mei-Zhong Luo, Rino Cella -- An end-trimming method and its application to amplify adjacent cDNA and genomic DNA fragments by PCR / Hiroyuki Iwahana, Mitsuo Itakura -- Anchoring a defined sequence to the 5' ends of mRNAs : the bolt to clone rare full-length mRNAs / Jean Baptiste Edwards, Olivier Valdenaire, Jacques Mallet -- Rapid directional walk within DNA clones by step-out PCR / Umadevi V. Wesley, Cedric S. Wesley -- Inverse PCR : an efficient approach to cloning cDNA ends / Sheng-He Huang -- Rapid amplification of gene ends (RAGE) from gene libraries by anchored PCR / Sheng-He Huang, Ambrose Y. Jong -- Isolation of coding sequences from yeast artificial chromosome (Yac) : clones by exon amplification / Fernando Gibson, Steve D. Brown -- cDNA libraries from a low amount of cells / Philippe Ravassard, Christine Icard-Liepkalns, Jacques Mallet, Jean Baptiste Edwards -- Rapid and nonradioactive screening of recombinant libraries by PCR / Michael W. King -- Use of PCR for cDNA library screening / Toru Takumi -- Generation and PCR screening of bacteriophage [lambda] sublibraries enriched for rare clones (the "sublibrary method") / Michael Lardelli -- Normalization of cDNA sequence representation by molecular selection / Thierry G. Coche -- Subtractive cDNA cloning using magnetic beads and PCR / Thierry G. Coche -- Generation of a PCR-renewable source of subtractive cDNA / W. Michael Kuehl, James Battey -- The use of PCR for differential screening of cDNA libraries / Mark G. Thomas, Sarah A. Hesse, Yvonne J. Foss, Farzin Farzaneh -- Identification and cloning of differentially expressed genes by DDRT-PCR / Mikkel Rohde, Rene Hummel, Niels Pallisgaard, Tino Podstufka, Heidi Riedel, Henrik Leffers, Michael Strauss -- Cloning gene family members using PCR with degenerate oligonucleotide primers / Gregory M. Preston -- Amplification using degenerate primers with multiple inosines to isolate genes with minimal sequence similarity / Simona Bartl -- Designing PCR primers to amplify specific members or subgroups of multigene families / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Screening gene family-enriched cDNA sublibraries with an unamplified cDNA probe : focusing on moderately to abundantly expressed clones / Meimei Hu, Bruce A. White
Control code
35192222
Dimensions
24 cm
Extent
xiv, 490 pages
Isbn
9780896034839
Lccn
96030876
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
System control number
(OCoLC)35192222

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