The Resource MUPrimer : a tool for finding allele specific PCR-primers for homologous gene sequences, M Mursaleen Ahmad, (electronic resource)

MUPrimer : a tool for finding allele specific PCR-primers for homologous gene sequences, M Mursaleen Ahmad, (electronic resource)

Label
MUPrimer : a tool for finding allele specific PCR-primers for homologous gene sequences
Title
MUPrimer
Title remainder
a tool for finding allele specific PCR-primers for homologous gene sequences
Statement of responsibility
M Mursaleen Ahmad
Title variation
MU Primer
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Polymerase chain reaction (PCR) amplification of specific regions of DNA has proven to be a powerful tool in molecular biology research. Synthetic short oligonucleotide DNA sequences (primers) provide specificity for the reaction by annealing to the target template DNA and providing the substrate for polymerase chain extension during the PCR. Primers are generally designed by extracting sequence matches from a single input DNA target with particular base composition, length, and position within the input DNA target. However, when the template DNA is from complex genomes or in mixtures of target DNAs, primer picking must be more sophisticated to enable discrimination of closely related sequences in the PCR. MUPrimer is a program to design allele-specific PCR primers that can discriminate among highly homologous gene sequences. The input target DNA includes of closely related sequences, making this software unique. User inputs include designating a master gene, primer positions, included and excluded regions, product length, primer T[subscript-m], and maximum T[subscript-m] difference allowed between forward and reverse primers. The software analyzes the sequences and returns primer pairs with a score that captures potential specificity of the primer based on the position of mismatches within a primer. The output is visual, displaying primer positions within the master sequence, a score for each primer, and an alignment of selected primer with the non-target sequences. MUPrimer is capable of providing PCR primers that enable allele-specific PCR amplification of specific target genes when in the presence of highly similar target sequences
Cataloging source
MUU
http://library.link/vocab/creatorDate
1985-
http://library.link/vocab/creatorName
Ahmad, M. Mursaleen
Degree
M.S.
Dissertation year
2009.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1972-
http://library.link/vocab/relatedWorkOrContributorName
Cheng, Jianlin
http://library.link/vocab/subjectName
  • Polymerase chain reaction
  • Nucleotide sequence
Target audience
specialized
Label
MUPrimer : a tool for finding allele specific PCR-primers for homologous gene sequences, M Mursaleen Ahmad, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. Jianlin Cheng
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
557591577
Extent
1 online resource (vi, 56 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
MU: Access is limited to the campuses of the University of Missouri.
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)557591577
Label
MUPrimer : a tool for finding allele specific PCR-primers for homologous gene sequences, M Mursaleen Ahmad, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. Jianlin Cheng
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
557591577
Extent
1 online resource (vi, 56 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
MU: Access is limited to the campuses of the University of Missouri.
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)557591577

Library Locations

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