The Resource Isolation and characterization of bacterial symbionts from Crotalaria spectabilis grown on trichloroethene contaminated soil, by April Lyn Rocha, (electronic resource)

Isolation and characterization of bacterial symbionts from Crotalaria spectabilis grown on trichloroethene contaminated soil, by April Lyn Rocha, (electronic resource)

Label
Isolation and characterization of bacterial symbionts from Crotalaria spectabilis grown on trichloroethene contaminated soil
Title
Isolation and characterization of bacterial symbionts from Crotalaria spectabilis grown on trichloroethene contaminated soil
Statement of responsibility
by April Lyn Rocha
Creator
Subject
Genre
Language
eng
Summary
"The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants' ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its ability to metabolize TCE. Nodules from C. spectabilis grown on TCE contaminated and non-contaminated soils were used to isolate the bacterial symbiont. Some species of Crotalaria are known to host Methylobacterium nodulans and this unique symbiont may explain why only the C. spectabilis nodules metabolize TCE. However, methane and methanol enrichments from the nodules were not successful and led to other approaches of isolating and identifying the symbiont. Sequencing of the 16S rRNA gene amplified from crushed nodules identified the symbionts as members of the Mesorhizobium genus. Symbiotic bacteria were isolated from control and dosed nodules and isolates of potential symbionts were verified by comparing PCR products from nodules and isolates using DGGE analysis. Sequencing of the 16S rRNA gene from the isolate confirmed that the isolates are members of the genus Mesorhizobium. This bacterium is different from symbionts isolated from other Crotalaria species and represent a new bacterium capable of nodulating Crotalaria sp. Attempts to demonstrate TCE metabolism by the isolated symbionts were unsuccessful suggesting that TCE metabolism may depend on a partnership with the host plant"--Abstract, p. iii
Related
Cataloging source
UMR
http://library.link/vocab/creatorDate
1980-
http://library.link/vocab/creatorName
Rocha, April Lyn
Degree
M.S.
Dissertation year
2011.
Granting institution
Missouri University of Science and Technology
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • theses
http://library.link/vocab/subjectName
  • Crotalaria spectabilis
  • Nucleotide sequence
  • Trichloroethylene
  • Gel electrophoresis
Target audience
specialized
Label
Isolation and characterization of bacterial symbionts from Crotalaria spectabilis grown on trichloroethene contaminated soil, by April Lyn Rocha, (electronic resource)
Instantiates
Publication
Note
  • Vita
  • The entire thesis text is included in file
  • Title from title screen of thesis/dissertation PDF file (viewed November 30, 2011)
Bibliography note
Includes bibliographical references (pages 42-45)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
764656024
Dimensions
unknown
Extent
1 online resource (ix, 46 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations, digital, PDF file.
Specific material designation
remote
System control number
(OCoLC)764656024
System details
  • System requirements: Adobe Acrobat Reader; Internet browser
  • Mode of access: World Wide Web
Label
Isolation and characterization of bacterial symbionts from Crotalaria spectabilis grown on trichloroethene contaminated soil, by April Lyn Rocha, (electronic resource)
Publication
Note
  • Vita
  • The entire thesis text is included in file
  • Title from title screen of thesis/dissertation PDF file (viewed November 30, 2011)
Bibliography note
Includes bibliographical references (pages 42-45)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
764656024
Dimensions
unknown
Extent
1 online resource (ix, 46 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations, digital, PDF file.
Specific material designation
remote
System control number
(OCoLC)764656024
System details
  • System requirements: Adobe Acrobat Reader; Internet browser
  • Mode of access: World Wide Web

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