The Resource In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway, by Melissa Taylor-Holou, (electronic resource)

In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway, by Melissa Taylor-Holou, (electronic resource)

Label
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway
Title
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway
Statement of responsibility
by Melissa Taylor-Holou
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Nudt16, previously called X29, is known to be a metal-dependent decapping enzyme expressed in mammals and Xenopus . Nudt16 has been proposed to be the catalytic component of a previously unknown nuclear RNA degradation pathway that degrades nuclear-limited RNAs. However, nothing was known about the function of the protein in other species. This work proposes to examine whether the sequence and, more importantly, the RNA decapping function of the Nudt16 protein is conserved across evolution. The hypothesis tested here is that if the protein and decapping function are conserved then the proposed in vivo role of a nuclear pathway for RNA degradation is more likely to be true. The experiments demonstrate that the sequence of the Nudt16 protein is evolutionarily conserved across metazoans. Additionally, there is evidence for a gene duplication that occurred at the level of tetrapods resulting in a cytoplasmic protein lacking RNA decapping function. Functional analysis of putative orthologs of Nudt16protein from several divergent organisms demonstrated that the ability to act as an RNA decapping protein is well conserved. More complete characterization of the Nudt16 protein from Xenopus as well we several of the other organisms demonstrated that the protein functions as homodimer with up to two RNA binding sites that may act independently of one another. While metal is a required co-factor, characterization of additional orthologs demonstrated that there is broad RNA substrate specificity for RNA decapping in the presence of both Mg+2 and Mn+2. Identification of putative in vivo interacting partners of Nudt16 was performed using co-immunoprecipitations. Three proteins were identified but still need to be verified. Collectively, these data demonstrate that Nudt16 is conserved both in sequence and in vitro function across evolution and support the existence of a nuclear RNA degradation pathway for nuclear-limited RNAs in Metazoans
Cataloging source
MUU
http://library.link/vocab/creatorDate
1983-
http://library.link/vocab/creatorName
Taylor-Holou, Melissa J.
Degree
Ph. D.
Dissertation year
2009.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1964-
http://library.link/vocab/relatedWorkOrContributorName
Peculis, Brenda A.
http://library.link/vocab/subjectName
  • Nucleotide sequence
  • Metazoa
  • Xenopus
  • RNA
Target audience
specialized
Label
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway, by Melissa Taylor-Holou, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on Sept 10, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Dissertation advisor: Dr. Brenda Peculis
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
695114986
Extent
1 online resource (xi, 237 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)695114986
Label
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway, by Melissa Taylor-Holou, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on Sept 10, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Dissertation advisor: Dr. Brenda Peculis
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
695114986
Extent
1 online resource (xi, 237 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)695114986

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