The Resource GABA [subscript B] receptor subunits : their developmental profile, response to physiological manipulations, and intracellular targets, by Maya Gadhvi Purisai

GABA [subscript B] receptor subunits : their developmental profile, response to physiological manipulations, and intracellular targets, by Maya Gadhvi Purisai

Label
GABA [subscript B] receptor subunits : their developmental profile, response to physiological manipulations, and intracellular targets
Title
GABA [subscript B] receptor subunits
Title remainder
their developmental profile, response to physiological manipulations, and intracellular targets
Statement of responsibility
by Maya Gadhvi Purisai
Title variation
  • Gamma aminobutyric acid B receptor subunits
  • GABA B receptor subunits
  • GABA receptor subunits
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
GABA [subscript B] receptors are G protein-coupled receptors. They inhibit neuronal excitation by inhibiting high voltage activated calcium channels and activating potassium channels. It was recently discovered that functional GABA [subscript B] receptors arise from hetero-dimerization of two proteins, GB1 and GB2, formed from genes that share almost 35% sequence identity. Various isoforms of the GB1 subunit have been identified, with GB1a and GB1b being most common. Not much is known about the expression of these receptors in either the pituitary (an endocrine organ) or the spinal cord (a part of the central nervous system) during development, a period marked with numerous critical changes. It is well established that GABA brings about excitation in neuronal transmission during early development via GABA [subscript A] receptors. However, little is known about the involvement of GABA [subscript B] receptors during this time. Thus, the present study was undertaken to determine the distribution pattern of these subunits and their isoforms in the pituitary and the spinal cord during development and adulthood. The role of GABA [subscript B] receptors in pain modulation is well established by behavioral studies; however, studies indicating alterations in gene expression as a result of pain stimulation are few. This dissertation work demonstrates a correlative relationship between GABA [subscript B] receptor expression and persistent pain. Using mIL tsA58 cells as an in vitro model of rat melanotrope cells, this dissertation work elucidates the signaling molecules associated with GABA [subscript B] receptors. This report also identifies the specific type of high voltage activated calcium channel modulated by GABA [subscript B] receptor activation in mIL tsA58 cells. Results indicate a differential expression of the receptor subunits and the GB1 isoforms in the pituitary and spinal cord during early development. There is an increase in GABA [subscript B] receptor mRNA and protein in the spinal cord as a result of formalin administration with a differential increase in the expression of GB1 isoforms. Furthermore, in mIL tsA58 cells, GABA [subscript B] receptors primarily connect with G0Ü type of G proteins to inhibit L-type high voltage activated calcium channels
Additional physical form
Available also on the Internet;
Cataloging source
UMK
http://library.link/vocab/creatorDate
1971-
http://library.link/vocab/creatorName
Purisai, Maya Gadhvi
Degree
Ph. D.
Dissertation year
2002.
Granting institution
School of Biological Sciences. University of Missouri--Kansas City
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Chronwall, Bibie M.
http://library.link/vocab/subjectName
  • GABA
  • Pituitary gland
  • Pain
  • Calcium channels
Label
GABA [subscript B] receptor subunits : their developmental profile, response to physiological manipulations, and intracellular targets, by Maya Gadhvi Purisai
Instantiates
Publication
Note
  • "A dissertation in cell biology and biophysics and molecular biology and biochemistry."
  • Advisor: Bibie M. Chronwall
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 140-162)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Contents
Overall objectives -- Background. GABA receptors ; High voltage activated-calcium channels ; Heterotrimeric G proteins ; The pituitary ; Melanotropes ; Melantrotrope development ; The spinal cord ; Spinal cord development ; Spinal cord and pain -- Materials and methods. Animals ; Tissue preparation ; Immunohistochemistry ; In situ hybridization ; In situ hybridization combined with immunohistochemistry ; Western blot assays ; Enzymatic deglycosylation of total protein extracts ; Reverse transcriptase-polymerase chain reaction ; Ribonuclease protection assay ; Characteristics of mIL cells ; Simultaneous measurement of intracellular calcium and pH levels ; Antisense deoxyoligonucleotide synthesis and administration -- Experimental results. To describe the cellular expression pattern of GABA [subscript B] receptor subunits and isoforms in the rat pituitary during development, aim 1 ; To describe the cellular expression pattern of GABA [subscript B] receptor subunits and isoforms in the rat spinal cord during development, aim 2 ; To test the hypothesis that GABA [subscript B] receptor expression in the dorsal lumbar spinal cord changes in response to pain, aim 3 -- To identify the type of HVA-CCs and G protein/s associated with GABA [subscript B] receptors in mIL cells, aim 4 -- Discussion of results. To describe the cellular expression pattern of GABA [subscript B] receptor subunits and isoforms in the rat pituitary during development, aim 1 ; To test the hypothesis that GABA [subscript B] receptor expression in the dorsal lumbar spinal cord changes in response to pain, aim 3 -- The identify the type of HVA-CCs and G protein/s associated with GABA [subscript B] receptors in mIL cells, aim 4 -- Conclusions -- Appendix. Solutions
Control code
56106797
Dimensions
28 cm.
Dimensions
unknown
Extent
xv, 163 leaves
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Note
  • UMKC: Microfiche copy. Ann Arbor, Mich. : UMI, 2002. 2 microfiches : positive ; 11 x 15 cm. "30-42013."
  • UMKC: N-B 9850.
Other physical details
illustrations
Specific material designation
remote
Label
GABA [subscript B] receptor subunits : their developmental profile, response to physiological manipulations, and intracellular targets, by Maya Gadhvi Purisai
Publication
Note
  • "A dissertation in cell biology and biophysics and molecular biology and biochemistry."
  • Advisor: Bibie M. Chronwall
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 140-162)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Contents
Overall objectives -- Background. GABA receptors ; High voltage activated-calcium channels ; Heterotrimeric G proteins ; The pituitary ; Melanotropes ; Melantrotrope development ; The spinal cord ; Spinal cord development ; Spinal cord and pain -- Materials and methods. Animals ; Tissue preparation ; Immunohistochemistry ; In situ hybridization ; In situ hybridization combined with immunohistochemistry ; Western blot assays ; Enzymatic deglycosylation of total protein extracts ; Reverse transcriptase-polymerase chain reaction ; Ribonuclease protection assay ; Characteristics of mIL cells ; Simultaneous measurement of intracellular calcium and pH levels ; Antisense deoxyoligonucleotide synthesis and administration -- Experimental results. To describe the cellular expression pattern of GABA [subscript B] receptor subunits and isoforms in the rat pituitary during development, aim 1 ; To describe the cellular expression pattern of GABA [subscript B] receptor subunits and isoforms in the rat spinal cord during development, aim 2 ; To test the hypothesis that GABA [subscript B] receptor expression in the dorsal lumbar spinal cord changes in response to pain, aim 3 -- To identify the type of HVA-CCs and G protein/s associated with GABA [subscript B] receptors in mIL cells, aim 4 -- Discussion of results. To describe the cellular expression pattern of GABA [subscript B] receptor subunits and isoforms in the rat pituitary during development, aim 1 ; To test the hypothesis that GABA [subscript B] receptor expression in the dorsal lumbar spinal cord changes in response to pain, aim 3 -- The identify the type of HVA-CCs and G protein/s associated with GABA [subscript B] receptors in mIL cells, aim 4 -- Conclusions -- Appendix. Solutions
Control code
56106797
Dimensions
28 cm.
Dimensions
unknown
Extent
xv, 163 leaves
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Note
  • UMKC: Microfiche copy. Ann Arbor, Mich. : UMI, 2002. 2 microfiches : positive ; 11 x 15 cm. "30-42013."
  • UMKC: N-B 9850.
Other physical details
illustrations
Specific material designation
remote

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