The Resource Dicer interacts with the P-granule component GLH-1 : both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules, by Erica Leigh Beshore, (electronic resource)

Dicer interacts with the P-granule component GLH-1 : both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules, by Erica Leigh Beshore, (electronic resource)

Label
Dicer interacts with the P-granule component GLH-1 : both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules
Title
Dicer interacts with the P-granule component GLH-1
Title remainder
both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules
Statement of responsibility
by Erica Leigh Beshore
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
P granules are ribonucleoprotein complexes specific to the cytoplasmic side of the nuclear pores of C. elegans germ cells. While P granules are implicated in post-transcriptional control of maternally-transcribed mRNAs, their function remains elusive. Our laboratory is particularly interested in the P-granule component GLH-1 (Germline RNA Helicase-1). Through genetic studies our laboratory has shown that GLH-1 is essential for fertility; however, the biochemical function of the GLH complex is still unknown. With immunoprecipitations and GST-pulldowns, we report that GLH-1 and the riboendonuclease Dicer bind one another and their interaction is not RNA dependent. Both GLH-1 protein and mRNA levels are reduced in the dcr- 1(ok247) mutant background; conversely, a reduction of DCR-1 protein in the glh-1(gk100) deletion strain is also observed. Thus, in complex in the C. elegans germline, these two proteins seem interdependent. In addition, evidence indicates Dicer protein levels, like those of GLH-1, are regulated by proteosomal degradation and are much increased when the Jun N-terminal kinase KGB-1 is missing in the kgb-1(um3) null. In the adult C. elegans germline DCR-1 is located throughout the cytoplasm as well as at the inner nuclear pores of the germ cell nuclei, in close opposition to GLH-1. Under stress conditions in oocytes GLH-1 and DCR-1 both re-locate and recruit other components to large cytoplasmic RNP granules. We hypothesize the GLH-1/DCR-1 complex may function in the transport, deposition, or regulation of maternally-transcribed mRNAs perhaps with their associated miRNAs
Cataloging source
MMU
http://library.link/vocab/creatorDate
1982-
http://library.link/vocab/creatorName
Beshore, Erica Leigh
Degree
Ph. D.
Dissertation year
2010.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
NLM call number
QU 56
http://library.link/vocab/relatedWorkOrContributorName
Bennett, Karen L.
http://library.link/vocab/subjectName
  • Nucleoproteins
  • Caenorhabditis elegans
  • Cytoplasmic granules
  • JNK mitogen-activated protein kinases
  • Germ cells
  • Messenger RNA
  • DEAD-box RNA Helicases
  • Ribonuclease III
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Cytoplasmic Granules
  • Germ Cells
  • JNK Mitogen-Activated Protein Kinases
  • Caenorhabditis elegans
  • RNA, Messenger
  • Ribonucleoproteins
Target audience
specialized
Label
Dicer interacts with the P-granule component GLH-1 : both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules, by Erica Leigh Beshore, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from PDF of title page (University of Missouri--Columbia, viewed on August 3, 2010)
  • Vita
  • Thesis advisor: Karen Bennett
  • "May 2010"
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
653299332
Extent
1 online resource (xiii, 168 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)653299332
Label
Dicer interacts with the P-granule component GLH-1 : both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules, by Erica Leigh Beshore, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from PDF of title page (University of Missouri--Columbia, viewed on August 3, 2010)
  • Vita
  • Thesis advisor: Karen Bennett
  • "May 2010"
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
653299332
Extent
1 online resource (xiii, 168 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)653299332

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