The Resource Association of the P6 protein of cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins, by Andres Rodriguez

Association of the P6 protein of cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins, by Andres Rodriguez

Label
Association of the P6 protein of cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins
Title
Association of the P6 protein of cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins
Statement of responsibility
by Andres Rodriguez
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
The P6 protein of Cauliflower mosaic virus (CaMV) is responsible for the formation of inclusion bodies (IBs), which are the site for viral gene expression, replication and virion assembly. Moreover, recent evidence indicates that ectopically expressed P6 IBs move in association with actin microfilaments. Since CaMV virions accumulate preferentially in P6 IBs, we hypothesized that P6 IBs have a role in delivering CaMV virions to the plasmodesmata. We recently discovered that the P6 protein interacted with a C2 calcium-dependent membrane targeting protein (designated AtSRC2-2) in a yeast two-hybrid screen and confirmed this interaction through co-immunoprecipitation and co-localization assays in the CaMV host, Nicotiana benthamiana. An AtSRC2-2 protein fused to RFP was localized to the plasma membrane and specifically associated with plasmodesmata. The AtSRC2-2-RFP fusion also co-localized with two proteins previously shown to associate with plasmodesmata: the host protein PDLP1 and the CaMV movement protein (MP). Since P6 IBs were found to co-localize with AtSCR2-2 and had previously been shown to interact with CaMV MP, we investigated whether a portion of the P6 IBs might also be associated with plasmodesmata. We examined the co-localization of P6-GFP IBs with PDLP1, the CaMV MP, and with aniline blue, a chemical stain for callose, and found that P6-GFP IBs were associated with each of these markers. Furthermore, a P6-RFP protein was co-immunoprecipitated with PDLP1-GFP. Our evidence that a portion of P6-GFP IBs associate with AtSRC2-2, PDLP1, and CaMV MP at plasmodesmata supports a model in which P6 IBs function to transfer CaMV virions directly to plasmodesmata
Cataloging source
MUU
http://library.link/vocab/creatorDate
1984-
http://library.link/vocab/creatorName
Rodriguez, Andres
Degree
Ph.D.
Dissertation note
Dissertation
Dissertation year
2014.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1958-
http://library.link/vocab/relatedWorkOrContributorName
Schoelz, James E.
Label
Association of the P6 protein of cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins, by Andres Rodriguez
Instantiates
Publication
Note
  • "May 2014."
  • "A Dissertation Presented to The Faculty of the Graduate School At the University of Missouri In Partial Fulfillment Of the Requirements for the Degree PhD in Plant, Insect and Microbial Sciences."
  • Dissertation Supervisor: Dr. James E. Schoelz
  • Includes vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
906039406
Extent
1 online resource (3 files)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)906039406
Label
Association of the P6 protein of cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins, by Andres Rodriguez
Publication
Note
  • "May 2014."
  • "A Dissertation Presented to The Faculty of the Graduate School At the University of Missouri In Partial Fulfillment Of the Requirements for the Degree PhD in Plant, Insect and Microbial Sciences."
  • Dissertation Supervisor: Dr. James E. Schoelz
  • Includes vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
906039406
Extent
1 online resource (3 files)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)906039406

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