The Resource A novel PCR based DNA microanalyzer system for detection of viral genome, by Shantanu Bhattacharya, (electronic resource)

A novel PCR based DNA microanalyzer system for detection of viral genome, by Shantanu Bhattacharya, (electronic resource)

Label
A novel PCR based DNA microanalyzer system for detection of viral genome
Title
A novel PCR based DNA microanalyzer system for detection of viral genome
Statement of responsibility
by Shantanu Bhattacharya
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
A micro-fluidic assay to quickly analyze microscopic samples of DNA is being developed for field applications. It consists of a micro-PCR chamber, micropumps, and micro-heaters. Additional components of the device include gel electrophoresis microchannels and solid core waveguide fluorescence collectors. The intended analyzer is a micro-fluidic platform that is principally based on the three-step polymerase chain reaction (PCR) mechanism. Currently, all off chip control is executed using a labview code. The micro-pumps, PCR chamber and capillary electrophoresis system have been designed fabricated and tested. For fabrication of the device, a regime has been developed for bonding PDMS surfaces to a variety of substrates (silicon in the present case). We have successfully achieved a compression in the cycle time by a factor of ten in our on chip PCR reactor as compared to the conventional PCR system and also amplify samples with pico-gram concentration. Fluorescent studies indicate negligible non-specific binding to our chip which has been a major problem in earlier assays. A working electrophoretic capillary and new biphasic gel material with extremely low background and high signal to noise ratio have been developed. We have further achieved low voltage capillary electrophoresis by doping different gel materials with conducting nano-particles. We envision this assay as a highly sensitive field deployable analyzer tool
Cataloging source
MUU
http://library.link/vocab/creatorDate
1974-
http://library.link/vocab/creatorName
Bhattacharya, Shantanu
Degree
Ph.D.
Dissertation year
2006.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
  • Gangopadhyay, Shubhra
  • Grant, Sheila Ann
http://library.link/vocab/subjectName
  • Polymerase chain reaction
  • DNA
  • Microchemistry
Target audience
specialized
Label
A novel PCR based DNA microanalyzer system for detection of viral genome, by Shantanu Bhattacharya, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (April 25, 2007)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
123497011
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)123497011
System details
Mode of access: World Wide Web
Label
A novel PCR based DNA microanalyzer system for detection of viral genome, by Shantanu Bhattacharya, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (April 25, 2007)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
123497011
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)123497011
System details
Mode of access: World Wide Web

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